Current Issue : April-June Volume : 2012 Issue Number : 2 Articles : 5 Articles
A new simple, precise, accurate and cost effective UV spectrophotometric method has been developed for the estimation of Cefpodoxime and Ofloxacin in combined dosage form by simultaneous equation method. This method utilized methanol as common solvent. The wavelength selected for simultaneous equation method for Cefpodoxime shows λmax 235nm and for Ofloxacin shows λmax 298nm. The drugs follow the Beer-Lamberts law in the concentration range of 2-10mcg/ml. The method was validated by following analytical parameters as suggested by the ICH guideline which included accuracy, precision, assay LOD and LOQ. All validation parameters were within acceptable range. The correlation coefficient of Cefpodoxime and Ofloxacin was found to be 0.9995 and 0.999 respectively. Recovery studies for Cefpodoxime 103.56 % and 102.58% for Ofloxacin in case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis....
First order derivative spectroscopy method involves the measurement of absorbances at zero crossing point of other drug. A new, rapid, precise, accurate and sensitive first derivative spectrophotometric method is proposed for the simultaneous estimation of Omeprazole and Cinitapride Hydrogen Tartrate in combined dosage form. The drugs obeyed the Beer’s law in the concentration range of 1-20 ug/ml (r2 = 0.9996 and r2 = 0.999) for Omeprazole and Cinitapride Hydrogen Tartrate both. In the proposed method, absorbance was measured at 236.5 nm (Zero Crossing Point of Cinitapride) and 275 nm (Zero Crossing Point of Omeprazole). Accuracy of the method was determined by recovery studies and the average percentage recovery was found to be 98.55% and 100.44% for Omeprazole and Cinitapride respectively. The LODs for Omeprazole and Cinitapride Hydrogen Tartrate were found to be 0.298 μg/ml and 0.425 μg/ml & LOQs were found to be 0.881μg/ml and 1.259 μg/ml respectively. Statistical analysis proves that the method is selective and sensitive for estimation of Omeprazole and Cinitapride Hydrogen Tartrate in combined dosage form. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline....
Today synthetic indicators are the choice of acid-base titrations. But due to environmental pollution, availability and cost, the search for natural compounds as an acid-base indicator was started. The present vocation highlights the exploit of the acidified ethanolic extract of the fruits of few medicinal plants as an acid-base indicator in acid-base titrations. This natural indicator is easy to extract as well as easily available. Promising results were obtained when it was tested against standard synthetic indicators. Titration shows sharp color change at the equivalence point. The equivalence points obtained by the fruits extract coincide with the equivalence points obtained by standard indicators. In case of weak acid and weak base titration, the results obtained by the fruits extract matched with the results obtained by mixed indicator. This natural indicator is found to be a very useful, economical, simple and accurate for the said titrations....
Two simple, rapid and economical ‘Zero Order UV - Spectrophotometric (Method I)’ and ‘First Order Derivative Spectrophotometric (Method II)’ using Area Under Curve (AUC) technique were developed for estimation of Cinitapride Hydrogen Tartrate (CNT) in tablet dosage form. In methanol, CNT showed maximum absorbance at 265 nm. In method I, AUC between two wavelengths 260 nm to 270 nm were selected. In method II, UV spectrum of CNT was derivatized into first order and two wavelengths 248 nm to 258 nm were selected for determination of AUC. In both these methods, CNT obeyed linearity in the concentration range of 1-20 μg/ml (r2 = 0.9995) and (r2 = 0.9992). Proposed methods were applied for tablet formulation and amounts of CNT estimated by Method I and Method II were found to be 99.29 + 1.07 and 101.87 + 1.84 respectively. Both these methods were validated statistically and by recovery experiment....
A simple, novel, sensitive, and specific spectrophotometric method was developed and validated for the determination of Paracetamol and Tolperisone Hydrochloride in bulk and its combined dosage form. First order derivative spectroscopy method is adopted to eliminate spectral interference. The method obeys Beer’s Law in concentration ranges selected for evaluation. Paracetamol and tolperisone hydrochloride have λmax at 248nm and 255 nm respectively in methanol. The method was validated for linearity, accuracy and precision as pe ICH norms. The zero cross over point for paracetamol and tolperisone hydrochloride was 248.6nm and 255nm respectively.The LOD and LOQ value were found to be 0.48 and 1.58 ppm for paracetamol and 0.86 and 2.87 ppm for tolperisone hydrochloride respectively. The developed and validated method was successfully used for the quantitative analysis of commercially available dosage form....
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